- Bacterial Cell Wall - an overview | ScienceDirect Topics
- Three-dimensional structure of the bacterial cell wall peptidoglycan
- First look at the structure of bacterial cell walls
- The Cell Wall of Bacteria
These data have been obtained in support of the hypothesis that penicillins are substrate analogues of the d -alanyl- d -alanine end of the nascent peptidoglycan units and that they acylate the transpeptidase which catalyzes the cross-linking reaction. The data may also provide an explanation of the paradoxical observation that the killing rate in S.
In the presence of low concentrations a weakened wall may be formed, thus rendering the organisms more susceptible to lysis than at high concentrations of penicillin where wall synthesis abruptly ceases.
Bacterial Cell Wall - an overview | ScienceDirect Topics
You'll be in good company. Journal of Lipid Research. Tipper and Jack L. Abstract An uncross-linked monomer nascent peptidoglycan unit accumulates in cells of Staphylococcus aureus treated with low concentrations of penicillin G or with ampicillin, methicillin, or cephalothin. Prev Next Table of Contents.
Services Email this article to a friend Alert me when this article is cited Alert me if a correction is posted Alert me when eletters are published Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Download to citation manager Request Permissions. Parameters for the carbohydrate portion of the molecule were obtained from glycam04 , which is a specialized set of parameters, derived for the accurate simulation of carbohydrates. The rest were treated with parameters from the parm99 data set that is part of the amber 7 suite of programs For the simulated annealing runs, the solvent was treated implicitly as a dielectric continuum by using the Generalized-Born approach 50 , An initial annealing simulation was carried out for several 5-ns periods at 2, K, and a series of snapshots served as initial coordinates for subsequent annealing runs.
A total of simulated annealing trajectories were carried out. Each consisted of three rounds of the following sequence.
The molecule was heated to 2, K for 10 ps, followed by 25 ps of dynamics at 2, K, and 25 ps of cooling to 0 K. The final cooling step was carried out for ps. This sequence gave a total of ps for each run and a combined ns of simulation for all trajectories. Each conformer was subjected to 10, steps of conjugate gradient energy minimization upon completion of the annealing run, and restraint violation energy was determined for each of the resulting energy-minimized structures.
The structures were ranked based on the restraint violation energy. The 50 conformers with the smallest violation energy were superimposed along the carbon and oxygen atoms of the saccharide rings. To construct the structure of [NAG-NAM] 8 from that of the experimentally determined 3D structure of compound 1 , we used the program sybyl 7.
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At each step this was followed by an energy minimization using the sybyl tripos force field with Gasteiger charges. The program pymol www. The structure was then docked into one of the small pores of the cell wall model by using sybyl 7. Twenty thousand steps of steepest decent energy minimization were subsequently carried out by using the tripos force field with Gasteiger charges, followed by a series of alternating short 10 ps molecular dynamics simulations and additional energy minimization steps to equilibrate the system. Author contributions: T. NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail.
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Three-dimensional structure of the bacterial cell wall peptidoglycan
Skip to main content. Three-dimensional structure of the bacterial cell wall peptidoglycan Samy O. Meroueh , Krisztina Z. Fisher , Timothy L. Stemmler , and Shahriar Mobashery. Samy O. Abstract The 3D structure of the bacterial peptidoglycan, the major constituent of the cell wall, is one of the most important, yet still unsolved, structural problems in biochemistry. Results and Discussion 3D Structure of the Peptidoglycan.
First look at the structure of bacterial cell walls
Methods NMR Spectroscopy. Computational Methods. NMR Data. Conflict of interest statement: No conflicts declared. J 79 : — , pmid: OpenUrl PubMed. Nishiyama Y. Soc : — , pmid: Klemm D. Ed 44 : — OpenUrl CrossRef. Ford Z. Res : — , pmid: Germer A. J 9 : — Aboitiz N. Colombo G. Formanek H. Biochem 46 : — , pmid: Burge R. Biol : — , pmid: Labischinski H.
The Cell Wall of Bacteria
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Goddard T. Wuthrich K. Case D. Bashford D. Chem 51 : — , pmid: Roux B. Previous Next. Back to top.
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